IMAGE PROCESSING AT ELLIPSOIDAL PHOTOMETRY
https://doi.org/10.21122/2220-9506-2016-7-1-67-76
Abstract
Ellipsoidal photometry using Charge-Coupled Device (CCD photometry), as a new kind of optical diagnostics of scattering media in reflected and/or transmitted light, requires the development of specific principles of data analysis. The object of this work was substantiation the principles of ellipsoidal CCD photometry at implementation of a new data processing method of spatial distribution of scattered optical flux. Procedure of photometric analysis include the steps of determining the image significance, as well as the size, configuration and illuminance in its respective areas with regard to the criteria to optimize the shape and sensitivity of the zone. Zone analysis schemes of photometric images for media with a radially symmetric and directed scattering are developed. Recommended to use the method of comparison with etalon for studies the technical surfaces and turbid media to determine their roughness and optical characteristics, respectively. During the analysis of biological media, there is a possible of prognosis the spatial distribution of the brightness of the image by means of statistical modeling of optical radiation in the system «biological medium + ellipsoidal reflector». That confirmed by comparing results of the numerical (direct Monte Carlo simulation) and the real experiment for samples different thickness of muscle porcine tissue in vitro. Parameters of the optical radiation, which used as input data for the simulation, correspond to a laser wavelength of 632.8 nm with a Gaussian distribution profile of power of 2 mW. As a CCD detector was used monochrome camera DMK-21Au04.AS, and ellipsoidal reflector with an eccentricity of 0.66 and working aperture of 33.75 mm. Obtained results of zone distribution of illumination across the field in real experimental photometric images during biometrics showed correlation with the total transmission, absorption and diffuse scattering coefficients. This interdependence may be important part of improvement inverse methods of determination the optical parameters of biological media.
About the Authors
M. A. BezuglyiUkraine
Address for correspondence: Bezuglyi M.A. National Technical University of Ukraine «Kyiv Polytechnic Institute», Peremohy Ave., 37, 03056, Kiev, Ukraine e-mail: mikhail_bezuglyy@ukr.net
N. V. Bezuglaya
Ukraine
A. B. Samilyak
Ukraine
References
1. Bezuglyi M.A., Bezuglaya N.V. Ellipsoidal reflectors in biomedical diagnostic. Proc. SPIE, 2013, р. 9032. 2. Romanishin W. An Introduction to Astronomical Photometry Using CCDs. University of Oklahoma, 2006, 175 p.
2. Warner B.D. A Practical Guide to Lightcurve Photometry and Analysis. New York: Springer-Verlag, 2006, 298 p.
3. Toporets A.S. Optikha sherohovatoj poverhnosti [Optics of rough surface]. Leningrad, Mashinostroenie Publ., 1988, 191 p. (in Russian).
4. Tuchin V.V. Light scaterring study of tissues. Physics-Uspekhi, 1997, vol. 40(5), pp. 495–515.
5. Bashkatov A.N., Genina E.A., Tuchin V.V. Optical Properties of Skin and Subcutaneous Tissues: a review. Journal of Innovative Optical Health Sciences, 2011, no. 1, pp. 9–38.
6. Bashkatov A.N., Genina E.A., Tuchin V.V. Tissue Optical Properties / Chapter 5 in Handbook of Biomedical Optics, David A. Boas, Constantinos Pitris, and Nimmi Ramanujam (editors), Taylor & Francis Group, LLC, CRC Press Inc., 2011, pp. 67–100.
7. Bezuglyi M.A., Pavlovets N.V. Optical biometry of biological tissues by ellipsoidal reflectors. Proc. OSASPIE, 2013, pp. 8798.
8. Bezuglaya N.V., Bezuglyi M.A., Tymchik G.S., Vonsevich K.P. Vliyaniye osevoj anizotropii rasseyaniya biologicheskikh sred na tochnost’ opredelenija koyefficientov metodom Monte-Karlo [The influence of the axial anisotropy of the scattering by biological media on the accuracy of determination the optical coefficients by Monte Carlo method]. Research Bulletin of NTUU «KPI», 2015, no. 1, pp. 85–91 (in Ukrainian).
9. Kryvoshlykov A.Yu., Sahno S.P., Smirnov V.A., Tymchik G.S. Cifrovaya korrekciya iskazhionnogo videosignala PZS-priyomnika [Digital correction of distorted video CCD detector] Bulletin «KPI». Series Instrument Making, 1986, no.16, pp. 59–62 (in Russian).
10. Cheong W.F., Prahl S.A., Welch A.J. A review of optical properties of biological tissues. Journal of quantum electronics, 1990, no. 12, pp. 2166–2239.
11. Meglinski I.V. Modeling the reflectance spectra of the optical radiation for random inhomogeneous multilayered highly scattering and absorbing media by the Monte Carlo technique. Quantum Electron, 2011, no. 31, pp. 1101–1107.
Review
For citations:
Bezuglyi M.A., Bezuglaya N.V., Samilyak A.B. IMAGE PROCESSING AT ELLIPSOIDAL PHOTOMETRY. Devices and Methods of Measurements. 2016;7(1):67-76. (In Russ.) https://doi.org/10.21122/2220-9506-2016-7-1-67-76