pimiПриборы и методы измеренийDevices and Methods of Measurements2220-95062414-0473BNTU10.21122/2220-9506-2022-13-2-93-104pimi-767Research ArticleСредства измеренийMeasuring instrumentsСпектрофотометры диффузного отражения на основе мини-спектрометров C12880MA и C11708MA HamamatsuDiffuse Reflectance Spectrophotometers Based on C12880MA and C11708MA Mini-Spectrometers HamamatsuФирагоB. А.FiragoV. A.

Address for correspondence: Firago V.A. - Belarusian State University, Nezavisimostу ave., 4, Minsk 220030, Belarus e-mail: firago@bsu.by

Адрес для переписки: Фираго В.А. – Белорусский государственный университет, пр-т Независимости, 4, г. Минск 220030, e-mail: firago@bsu.by

firago@bsu.byЛевковичН. В.LevkovichN. V.

Nezavisimostу ave., 4, Minsk 220030, Belarus

пр-т Независимости, 4, г. Минск 220030

ШуликоК. И.ShulikoK. I.

Nezavisimostу ave., 4, Minsk 220030, Belarus

пр-т Независимости, 4, г. Минск 220030

Белорусский государственный университетБеларусьBelarusian State UniversityBelarus20220607202213293104Copyright © Фираго B.А., Левкович Н.В., Шулико К.И., 20222022Фираго B.А., Левкович Н.В., Шулико К.И.Firago V.A., Levkovich N.V., Shuliko K.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://pimi.bntu.by/jour/article/view/767

Спектроскопия диффузного отражения с пространственным разрешением – перспективное направление неразрушающего контроля свойств ряда рассеивающих мелкодисперсных материалов, в том числе и продуктов питания. Она может найти широкое практическое применение только при наличии компактной, простой в применении и недорогой спектрофотометрической техники. Цель статьи – исследование возможности создания портативных спектрофотометров на основе мини-спектрометров Hamamatsu, которые работают в комплексе с современными вычислительными средствами.

Рассмотрены схемы подключения мини-спектрометров C12880MA и C11708MA к портативным вычислительным устройствам. Показана целесообразность использования малогабаритной микропроцессорной платы ARM STM32F103C8T6 (Blue pill) на чипе STM32F103C8T6. Её использование в схеме подключения позволило упростить обмен данными с управляющим компьютером по USB интерфейсу и формирование всех сигналов, которые необходимы для управления работой мини-спектрометра.

Созданы два экспериментальных образца спектрофотометров на основе мини-спектрометров C12880MA и C11708MA и микропроцессоров STM32 и исследованы их характеристики. Приведены методика градуировки и особенности программного обеспечения этих спектрофотометров. Описанные особенности обеспечивают оперативность модификации программного обеспечения под решаемую спектрофотометрическую задачу. Выявлено наличие искажений регистрируемых спектров в коротковолновом участке спектрального диапазона C12880MA. Они возникают за счёт фокусировки вогнутой дифракционной решеткой части рассеиваемого ей излучения в нулевой порядок.

Апробация созданных портативных спектрофотометров на основе мини-спектрометров Hamamatsu указывает на возможность их применения в портативной спектрофотометрической технике и устройствах спектрального контроля оптических свойств рассеивающих материалов. Описанная методика градуировки позволяет определять диапазон спектра, в котором искажения регистрируемых спектров минимальны. Предлагаемые решения позволяют существенно снизить стоимость устройств спектроскопии диффузного отражения с пространственным разрешением и расширить возможности их использования в различных отраслях науки и производства.

Diffuse reflection spectroscopy with spatial resolution is a promising direction of non-destructive control of the properties of a number of scattering fine-dispersed materials, including food products. It can find wide practical application only in the presence of compact, easy-to-use and inexpensive spectrophotometric equipment. The aim of the article is to investigate the possibility of creating portable spectrophotometers based on Hamamatsu mini-spectrometers, which work together with modern computing facilities.

The schematics for connecting the C12880MA and C11708MA mini-spectrometers to portable computing devices are reviewed. Shows the feasibility of using a small-sized microprocessor board ARM STM32F103C8T6 (Blue pill) on the chip STM32F103C8T6. Its use in the connection scheme has simplified data exchange with the control computer via USB interface and the formation of all the signals that are needed to control the mini-spectrometer.

Two experimental samples of spectrophotometers based on C12880MA and C11708MA mini-spectrometers and STM32 microprocessors were created and their characteristics were studied. The calibration procedure and features of the software for these spectrophotometers are presented. The described features ensure the efficiency of software modification for the spectrophotometric problem to be solved. The presence of distortions of the registered spectra in the short-wave part of the spectral range of C12880MA was revealed. They arise due to focusing by the concave diffraction grating of a part of the radiation scattered by it into zero order.

Approbation of developed portable spectrophotometers based on Hamamatsu mini-spectrometers indicates the possibility of their use in portable spectrophotometric equipment and devices for spectral control of optical properties of scattering materials. The described calibration technique allows you to determine the range of the spectrum, in which the distortions of the recorded spectra are minimal. The proposed solutions significantly reduce the cost of diffuse reflectance spectroscopy devices with spatial resolution and expand the possibilities of their use in various branches of science and industry.

диффузное отражениемини-спектрометрыспектральная чувствительностьспектрофотометрыградуировка спектрометровdiffuse reflectionmini-spectrometersspectral sensitivityspectrophotometerscalibration of spectrometers.ReferencesPuig-Bertotto J., Coelloa J., Maspoch S. Evaluation of a handheld near-infrared spectrophotometer for quantitative determination of two APIs in a solid pharmaceutical preparation. Analytical Methods, 2019, vol. 11, iss. 3, pp. 327–335. DOI: 10.1039/C8AY01970CPuig-Bertotto J., Coelloa J., Maspoch S. Evaluation of a handheld near-infrared spectrophotometer for quantitative determination of two APIs in a solid pharmaceutical preparation. Analytical Methods, 2019, vol. 11, iss. 3, pp. 327–335. DOI: 10.1039/C8AY01970CTang E. Rapid Analysis of Textiles with Portable Near Infrared Spectroscopy. NIR news, 2015, vol. 26, iss. 8, pp. 11–12. DOI: 10.1255/nirn.1566Tang E. Rapid Analysis of Textiles with Portable Near Infrared Spectroscopy. NIR news, 2015, vol. 26, iss. 8, pp. 11–12. DOI: 10.1255/nirn.1566Nicolaï B.M., Beullens K., Bobelyn E., Peirs A., Saeys W., Theron K.I., Lammertyn J. Nondestructive measurement of fruit and vegetable quality by means of NIR spectroscopy: A review. J. Postharvest Biology Technology, 2007, vol. 46, iss. 2, pp. 99–118. DOI: 10.1016/j.postharvbio.2007.06.024Nicolaï B.M., Beullens K., Bobelyn E., Peirs A., Saeys W., Theron K.I., Lammertyn J. Nondestructive measurement of fruit and vegetable quality by means of NIR spectroscopy: A review. J. Postharvest Biology Technology, 2007, vol. 46, iss. 2, pp. 99–118. DOI: 10.1016/j.postharvbio.2007.06.024Do Trong N.N., Erkinbaev C., Tsuta M., De Baerdemaeker J., Nicolaï B., Saeys W. Spatially resolved diffuse reflectance in the visible and near-infrared wavelength range for non-destructive quality assessment of “Braeburn” apples. Postharvest Biology and Technology, 2014, vol. 91, pp. 39–48. DOI: 10.1016/j.postharvbio.2013.12.004Do Trong N.N., Erkinbaev C., Tsuta M., De Baerdemaeker J., Nicolaï B., Saeys W. Spatially resolved diffuse reflectance in the visible and near-infrared wavelength range for non-destructive quality assessment of “Braeburn” apples. Postharvest Biology and Technology, 2014, vol. 91, pp. 39–48. DOI: 10.1016/j.postharvbio.2013.12.004Doornbos R.M., Lang R., Aalders M.C., Cross F.W., Sterenborg H.J.C.M. The determination of in vivo human tissue optical properties and absolute chromophore concentrations using spatially resolved steadystate diffuse reflectance spectroscopy. Phys. Med. Biol., 1999, vol. 44, no. 4, pp. 967–981. DOI: 10.1088/0031-9155/44/4/012Doornbos R.M., Lang R., Aalders M.C., Cross F.W., Sterenborg H.J.C.M. The determination of in vivo human tissue optical properties and absolute chromophore concentrations using spatially resolved steadystate diffuse reflectance spectroscopy. Phys. Med. Biol., 1999, vol. 44, no. 4, pp. 967–981. DOI: 10.1088/0031-9155/44/4/012Thueler P., Charvet I., Bevilacqua F., Ghislain M. In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties. J. Biomedical Optics, 2003, vol. 8, no. 3, pp. 495–503. DOI: 10.1117/1.1578494Thueler P., Charvet I., Bevilacqua F., Ghislain M. In vivo endoscopic tissue diagnostics based on spectroscopic absorption, scattering, and phase function properties. J. Biomedical Optics, 2003, vol. 8, no. 3, pp. 495–503. DOI: 10.1117/1.1578494Abookasis D., Zafrir E., Nesher E., Pinhasov A., Sternklar S. Diffuse near-infrared reflectance spectroscopy during heatstroke in a mouse model: pilot study. J. Biomedical Optics, 2012, vol. 17, no. 10, pp. 105009-1– 105009-11. DOI: 10.1117/1.JBO.17.10.105009Abookasis D., Zafrir E., Nesher E., Pinhasov A., Sternklar S. Diffuse near-infrared reflectance spectroscopy during heatstroke in a mouse model: pilot study. J. Biomedical Optics, 2012, vol. 17, no. 10, pp. 105009-1– 105009-11. DOI: 10.1117/1.JBO.17.10.105009Abookasis D., Shochat A., Marlon S. Monitoring hemodynamic and morphologic responses to closed head injury in a mouse model using orthogonal diffuse nearinfrared light reflectance spectroscopy. J. Biomedical Optics, 2013, vol. 18, no. 4, pp. 04503-1–04503-11. DOI: 10.1117/1.JBO.18.4.045003Abookasis D., Shochat A., Marlon S. Monitoring hemodynamic and morphologic responses to closed head injury in a mouse model using orthogonal diffuse nearinfrared light reflectance spectroscopy. J. Biomedical Optics, 2013, vol. 18, no. 4, pp. 04503-1–04503-11. DOI: 10.1117/1.JBO.18.4.045003Bevilacqua F., Piguet D., Marquet P., Gross J.D., Tromberg B.J., Depeursinge C. In vivo local determination of tissue optical properties: applications to human brain. Applied optics, 1999, vol. 38, no. 22, pp. 4939– 4950. DOI: 10.1364/ao.38.004939Bevilacqua F., Piguet D., Marquet P., Gross J.D., Tromberg B.J., Depeursinge C. In vivo local determination of tissue optical properties: applications to human brain. Applied optics, 1999, vol. 38, no. 22, pp. 4939– 4950. DOI: 10.1364/ao.38.004939Micro-spectrometr C12880MA. Hamamatsu Photonics K. K. Solid State Division. Cat. No. KACC1226E02. Oct. 2015 DN, pp. 1–12.Micro-spectrometr C12880MA. Hamamatsu Photonics K. K. Solid State Division. Cat. No. KACC1226E02. Oct. 2015 DN, pp. 1–12.Mini-spectrometers C10988MA-01, C11708MA. Hamamatsu Photonics K. K. Solid State Division. Cat. No. KACC1169E11. Oct. 2014 DN, pp. 1–10.Mini-spectrometers C10988MA-01, C11708MA. Hamamatsu Photonics K. K. Solid State Division. Cat. No. KACC1169E11. Oct. 2014 DN, pp. 1–10.Firago V.A., Sakovich I.A., Sabchuk A.N. [Fiber-optic spectrophotometer to register the spectra of thermal radiation of heated bodies]. Materialy 4 MNPK “Prikladnyye problemy optiki, informatiki, radiofiziki i fiziki kondensirovannogo sostoyaniya”. 11–12 maya 2017 g., Minsk [Materials of 4 ISPK “Applied problems of optics, informatics, radiophysics and condensed matter physics”. May 11–12, 2017, Minsk], pp. 148–150 (іn Russian).Firago V.A., Sakovich I.A., Sabchuk A.N. [Fiber-optic spectrophotometer to register the spectra of thermal radiation of heated bodies]. Materialy 4 MNPK “Prikladnyye problemy optiki, informatiki, radiofiziki i fiziki kondensirovannogo sostoyaniya”. 11–12 maya 2017 g., Minsk [Materials of 4 ISPK “Applied problems of optics, informatics, radiophysics and condensed matter physics”. May 11–12, 2017, Minsk], pp. 148–150 (іn Russian).Firago V., Hotra O., Sakovich I. Radiometric calibration of fiber optic spectrophotometers. Proceedings of SPIE – The International Society for Optical Engineering, 2019, vol. 11045, pp. 1104516-1– 1104516-7. DOI: 10.1117/12.2522540Firago V., Hotra O., Sakovich I. Radiometric calibration of fiber optic spectrophotometers. Proceedings of SPIE – The International Society for Optical Engineering, 2019, vol. 11045, pp. 1104516-1– 1104516-7. DOI: 10.1117/12.2522540Firago V.A. Tsifrovaya termografiya [Digital thermography]. Minsk, BSU Publ., 2019, 319 p.Firago V.A. Tsifrovaya termografiya [Digital thermography]. Minsk, BSU Publ., 2019, 319 p.

The authors declare that there are no conflicts of interest present.