pimiПриборы и методы измеренийDevices and Methods of Measurements2220-95062414-0473BNTU10.21122/2220-9506-2019-10-2-160-168pimi-439Research ArticleМетоды измерений, контроля, диагностикиMethods of measurements, monitoring, diagnosticsУгловая фотометрия биологических тканей методом эллипсоидальных рефлекторовAngular Photometry of Biological Tissue by Ellipsoidal Reflector MethodБезуглыйM. А.BezuglyiM. A.

Адрес для переписки: М.А. Безуглый – Национальный технический университет Украины «Киевский политехнический институт», пр. Победы, 37, г. Киев 03056, Украина     e-mail: mikhail_bezuglyy@ukr.net

Address for correspondence: M.A. Bezuglyi – National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», Peremohy Ave., 37, Kiev 03056, Ukraine     e-mail: mikhail_bezuglyy@ukr.net

mikhail_bezuglyy@ukr.netБезуглаяН. В.BezuglayaN. V.ВенцурикА. В.VentsurykA. V.ВонсевичК. П.VonsevychK. P.Национальный технический университет Украины «Киевский политехнический институт имени Игоря Сикорского»УкраинаNational Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute»Ukraine201924062019102160168Copyright © Безуглый M.А., Безуглая Н.В., Венцурик А.В., Вонсевич К.П., 20192019Безуглый M.А., Безуглая Н.В., Венцурик А.В., Вонсевич К.П.Bezuglyi M.A., Bezuglaya N.V., Ventsuryk A.V., Vonsevych K.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://pimi.bntu.by/jour/article/view/439

Угловые измерения в оптике биологических тканей широко применяются для решения различных прикладных спектроскопических задач для контроля шероховатой поверхности, определения показателя преломления, а также для изучения оптических свойств. Целью данной работы являлось исследование отражающей способности биологических тканей методом зеркальных эллипсоидов вращения при переменных углах падения зондирующего изучения.

В работе исследуются функциональные возможности усовершенствованного метода фотометрии эллипсоидальными рефлекторами. Разработана и представлена установка для фотометрии зеркальным эллипсоидом вращения в отраженном свете. Для обеспечения работы метода представлены теоретические основы конструирования эллипсоидального рефлектора с характерным пазом для обеспечения ввода лазерного излучения в исследуемую область. Получены аналитические выражения для вычисления диапазона углов падающего излучения в зависимости от эксцентриситета и фокального параметра эллипсоида. Также представлена усовершенствованная схема обработки изображений, получаемых при угловой фотометрии эллипсоидальными рефлекторами.

Представлены результаты серии экспериментальных исследований для мышечной ткани на длинах волн 405 нм, 532 нм и 650 нм. В ходе эксперимента были получены фотометрические изображения при использовании фотометра со следующими конструктивными параметрами: диапазон угла падения лазерного излучения 12.5–62.5°, эксцентриситет эллипсоидального рефлектора 0,6, фокальный параметр 18 мм и ширина паза 8 мм.

Характер светорассеяния мышечными тканями на различных длинах волн представлен графиками для зоны коллимированного отражения. При этом форма зоны падающего потока позволяет оценить влияние оптических свойств внутреннего или приповерхностного слоя биологической тканине на светорассеяние при различных углах падения.

Angular measurements in optics of biological tissues are used for different applied spectroscopic task for roughness surface control, define of refractive index and for research of optical properties. Purpose of the research is investigation of the reflectance of biologic tissues by the ellipsoidal reflector method under the variable angle of the incident radiation.

The research investigates functional features of improved photometry method by ellipsoidal reflectors. The photometric setup with mirror ellipsoid of revolution in reflected light was developed. Theoretical foundations of the design of an ellipsoidal reflector with a specific slot to ensure the input of laser radiation into the object area were presented. Analytical solution for calculating the angles range of incident radiation depending on the eccentricity and focal parameter of the ellipsoid are obtained. Also created the scheme of image processing at angular photometry by ellipsoidal reflector.

The research represents results of experimental series for samples of muscle tissues at wavelengths 405 nm, 532 nm, 650 nm. During experiment there were received photometric images on the equipment with such parameters: laser beam incident angles range 12.5–62.5°, ellipsoidal reflector eccentricity 0.6, focal parameter 18 mm, slot width 8 mm.

The nature of light scattering by muscle tissues at different wavelengths was represented by graphs for the collimated reflection area. The investigated method allows qualitative estimation of influence of internal or surface layers of biologic tissues optical properties on the light scattering under variable angles of incident radiation by the shape of zone of incident light.

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The authors declare that there are no conflicts of interest present.